Antibody Response in Chickens to Infection with Haemophilus gallinarum

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Abstract
H. gallinarum strains 0083 and 17756 were adapted to grow in broth consisting of brain heart infusion (Difco), DPN.H (Sigma), 25 [mu]g/ml, and horse serum, 1.0%.Yields of 5-6% of whole-cell plate agglutination antigen were obtained routinely in this medium. The antigens were sensitive, specific, and stable. Agglutinins were detected 7 to 14 days after development of coryza as a result of infection by intrasinus inoculation of H. gallinarum or by contact. Birds not showing frank disease after infection had a delayed antibody response. Such birds were as resistant to reinfection as birds which had recovered from the disease. A "healthy" carrier state was demonstrated in chickens which had recovered from H. gallinarum infection.