Development and application of organic reagents for analysis. VIII. Determination of biological thiols with a new fluorogenic thiol-selective reagent, N-{p-(2-(6-dimethylamino)benzofuranyl)phenyl}maleimide.

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Abstract
A method for the determination of thiol compounds is described. A new fluorogenic reagent, N-{p-[2-(6-dimethylamino)benzofuranyl]phenyl}maleimide, has been found to give fluorescent products when reacted with certain biological thiols, e.g., reduced glutathione and cysteine. The reaction is very sensitive, and concentrations as low as 10-9 M can be detected. Disulfides such as oxidized glutathione and cystine can also be determined after reduction of the disulfide to thiol with KBH4. The proposed method has been successfully applied to the determination of total thiol compounds in rat tissues and rat blood.