Transfer and expression of heterologous genes in yeasts other than Saccharomyces cerevisiae
- 3 October 2005
- book chapter
- Published by Springer Nature
- Vol. 43, 75-102
- https://doi.org/10.1007/bfb0009080
Abstract
In the past few years, yeasts other than those belonging to the genus Saccharomyces have become increasingly important for industrial applications. Species such as Pichia pastoris, Hansenula polymorpha, Schizosaccharomyces pombe, Yarrowia lipolytica and Kluyveromyces lactis have been modified genetically and used for the production of heterologous proteins. For a number of additional yeasts such as Schwanniomyces occidentalis, Zygosaccharomyces rouxii, Trichosporon cutaneum, Pachysolen tannophilus, Pichia guilliermondii and members of the genus Candida genetic transformation systems have been worked out. Transformation was achieved using either dominant selection markers based on antibiotic resistance genes or auxotrophic markers in conjunction with cloned biosynthetic genes involved in amino acid or nucleotide metabolism.Keywords
This publication has 218 references indexed in Scilit:
- Transformation of a glucose negative mutant ofPachysolen tannophilus with a plasmid carrying the cloned hexokinase PII gene fromSaccharomyces cerevisiaeBiotechnology Letters, 1989
- Expression of the SV40 promoter in fission yeast: Identification and characterization of an AP-1-like factorCell, 1988
- Invertase gene (SUC2) of Saccharomyces cerevisiae as a dominant marker for transformation of Pichia pastorisGene, 1987
- D-Xylose fermentation to ethanol bySchizosaccharomyces pombe cloned with xylose isomerase geneBiotechnology Letters, 1986
- The fos gene product undergoes extensive post-translational modification in eukaryotic but not in prokaryotic cellsGene, 1986
- An efficient chloramphenicol-resistance marker for Saccharomyces cerevisiae and Escherichia coliGene, 1986
- Yeast promoters URA1 and URA3Journal of Molecular Biology, 1985
- Transformation of Rhodosporidium toruloidesGene, 1985
- A positive selection for mutants lacking orotidine-5′-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistanceMolecular Genetics and Genomics, 1984
- Enrichment of auxotrophic mutants inHansenula polymorphaApplied Microbiology and Biotechnology, 1977