The protein that binds the 3' end of histone mRNA: a novel RNA-binding protein required for histone pre-mRNA processing.
Open Access
- 1 December 1996
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 10 (23) , 3028-3040
- https://doi.org/10.1101/gad.10.23.3028
Abstract
Replication-dependent histone mRNAs are not polyadenylated but end in a conserved 26-nucleotide structure that contains a stem-loop. Much of the cell cycle regulation of histone mRNA is post-transcriptional and is mediated by the 3' end of histone mRNA. The stem-loop binding protein (SLBP) that binds the 3' end of histone mRNA is a candidate for the factor that participates in most, if not all, of the post-transcriptional regulatory events. We have cloned the cDNA for the SLBP from humans, mice, and frogs, using the recently developed yeast three-hybrid system. The human SLBP is a 31-kD protein and contains a novel RNA-binding domain, which has been mapped to a 73-amino-acid region of the protein. The cloned SLBP is the protein bound to the 3' end of histone mRNA as antibodies specific for the SLBP remove all specific binding activity from nuclear and polyribosomal extracts. These depleted extracts do not cleave histone pre-mRNA efficiently, demonstrating that the SLBP is required for efficient histone pre-mRNA processing.Keywords
This publication has 28 references indexed in Scilit:
- Efficient Extraction and Partial Purification of the Polyribosome-Associated Stem−Loop Binding Protein Bound to the 3‘ End of Histone mRNABiochemistry, 1996
- PROTEIN-RNA RECOGNITIONAnnual Review of Biochemistry, 1995
- Mechanisms of mRNA degradation in eukaryotesTrends in Biochemical Sciences, 1994
- Expressed genes, Alu repeats and polymorphisms in cosmids sequenced from chromosome 4p16.3Nature Genetics, 1992
- Shuttling of pre-mRNA binding proteins between nucleus and cytoplasmNature, 1992
- Multiple processing-defective mutations in a mammalian histone pre-mRNA are suppressed by compensatory changes in U7 RNA both in vivo and in vitro.Genes & Development, 1991
- Do the poly(A) tail and 3′ untranslated region control mRNA translation?Cell, 1990
- Poly(A), poly(A) binding protein and the regulation of mRNA stabilityTrends in Biochemical Sciences, 1989
- Multiple regulatory steps control histone mRNA concentrationsTrends in Biochemical Sciences, 1988
- Identification of the Human U7 snRNP as One of Several Factors Involved in the 3′ End Maturation of Histone Premessenger RNA'sScience, 1987