Deletion of NH2−and COOH-terminal sequences destroys function of the Ca2+ATPase of rabbit fast-twitch skeletal muscle sarcoplasmic reticulum
- 20 December 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 336 (1) , 168-170
- https://doi.org/10.1016/0014-5793(93)81633-b
Abstract
Deletion mutants of the Ca2+ ATPase of rabbit fast‐twitch skeletal muscle sarcoplasmic reticulum (SERCA1a) were constructed and expressed in COS‐1 cells. The mutants were expressed at levels 7‐ to 15‐fold lower than the wild‐type and were inactive. In vitro transcription‐translation‐insertion experiments showed that deletion of transmembrane sequences M1 and M2, but not of M8, M9, M10 or the NH2− terminal 30 amino acids inhibited the stable insertion of the enzyme into the membrane. Thus there was no correlation between loss of function and membrane insertion. A signal sequence for membrane insertion may exist in M1 and M2.Keywords
This publication has 10 references indexed in Scilit:
- Metabolic rates of minke whales (Balaenoptera acutorostrata) in cold waterActa Physiologica Scandinavica, 1992
- Molecular tools to elucidate problems in excitation-contraction couplingBiophysical Journal, 1990
- Location of high affinity Ca2 +-binding sites within the predicted transmembrahe domain of the sarco-plasmic reticulum Ca2+-ATPaseNature, 1989
- Mutation of aspartic acid-351, lysine-352, and lysine-515 alters the Ca2+ transport activity of the Ca2+-ATPase expressed in COS-1 cells.Proceedings of the National Academy of Sciences, 1988
- Two Ca2+ ATPase genes: Homologies and mechanistic implications of deduced amino acid sequencesCell, 1986
- Amino-acid sequence of a Ca2+ + Mg2+ -dependent ATPase from rabbit muscle sarcoplasmic reticulum, deduced from its complementary DNA sequenceNature, 1985
- Human GM-CSF: Molecular Cloning of the Complementary DNA and Purification of the Natural and Recombinant ProteinsScience, 1985
- Rapid and efficient site-specific mutagenesis without phenotypic selection.Proceedings of the National Academy of Sciences, 1985
- Efficientin vitrosynthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoterNucleic Acids Research, 1984
- Polypeptide and phospholipid composition of the membrane of rat liver peroxisomes: comparison with endoplasmic reticulum and mitochondrial membranes.The Journal of cell biology, 1982