The sevenmaker gain‐of‐function mutation in p42 MAP kinase leads to enhanced signalling and reduced sensitivity to dual specificity phosphatase action
- 26 September 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 352 (2) , 201-205
- https://doi.org/10.1016/0014-5793(94)00958-9
Abstract
A mammalian mutant MAP kinase, D319N ERK2, analogous to Drosophila melanogaster sevenmaker (rlsem) gain-of-function mutation was shown to have an increased sensitivity to low levels of signalling in vivo. However, the mutation does not lead to an elevated basal kinase activity and still requires activation by MAP kinase kinase (MAPKK) as does wild type ERK2. This increased responsiveness seen in vivo is not due to an increased ability to phosphorylate substrates but appears to reflect a reduced sensitivity to a MAP kinase phosphatase CL100.Keywords
This publication has 43 references indexed in Scilit:
- Requirement for Ras in Raf activation is overcome by targeting Raf to the plasma membraneNature, 1994
- Genetics of signal transduction in invertebratesCurrent Opinion in Genetics & Development, 1994
- The SRF accessory protein Elk-1 contains a growth factor-regulated transcriptional activation domainCell, 1993
- cPLA2 is phosphorylated and activated by MAP kinaseCell, 1993
- Activation of the MAP kinase pathway by the protein kinase rafCell, 1992
- Oxidative stress and heat shock induce a human gene encoding a protein-tyrosine phosphataseNature, 1992
- Serum-, TPA-, and Ras-induced expression from Ap-1/Ets-driven promoters requires Raf-1 kinase.Genes & Development, 1992
- Phosphorylation of c-jun mediated by MAP kinasesNature, 1991
- Dissection of the protein kinase cascade by which nerve growth factor activates MAP kinasesNature, 1991
- The drosophila ras oncogenes: Structure and nucleotide sequenceCell, 1984