Abstract
Freshly isolated lenses incubated in either M-199 alone or supplemented with serum or insulin were measured for α- and γ-crystallin synthesis. It was found that synthesis of these crystallins were not altered by addition of either serum or insulin in the culture medium. The α-crystallin synthesis showed decreased synthetic activity after 1 day of culture, whereas γ-crystallin synthesis maintained a comparatively similar rate of synthesis for 2 days.

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