The Changes with Age in the Distribution of Copper and Some Copper-containing Oxidases in Red Clover (Trifolium pratenseL. cv. Dorset Marlgrass)
- 1 June 1973
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 24 (3) , 525-536
- https://doi.org/10.1093/jxb/24.3.525
Abstract
Changes in activity with age of four copper-containing enzymes-ascorbate oxidase, diamine oxidase, o-diphenol oxidase, and cytochrome oxidase-were studied in red clover plants (Trifolium pratense L. cv. Dorset Marlgrass) grown in nutrient solutions with or without copper. In the first 2 weeks after germination the cytochrome oxidase activities of plants grown with or without copper were similar, but the activities of the other three enzymes were less in plants grown without copper. At 6 weeks the activities of all four enzymes were less in plants grown without copper. The copper content of the cotyledons did not change during the first few weeks of growth suggesting that this copper is not easily mobilized. A new method for determining 1–10 ng amounts of Cu2+ is described based on the specific reactivation of the copper-free apoenzyme of pea-seedling diamine oxidase.Keywords
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