Mitochondria are associated with microtubules and not with intermediate filaments in cultured fibroblasts.

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Abstract
Triple-immunofluorescence experiments with antibodies to cytochrome c oxidase, tubulin, and vimentin were used to immunolabel the mitochondria, microtubules and intermediate filaments inside the same cultured fibroblasts [NRK and W138]. In particular, fibroblasts were immunolabeled after they had either been transformed by infection with Rous sarcoma virus or given long-term treatment with cycloheximide. These treatments induced redistribution of the intermediate filaments into a perinuclear arrangement, segregated away from the microtubules, which remained extended to the cell periphery. In such cells, many labeled mitochondria were codistributed with the peripherally located microtubules. An association, probably involving some type of chemical linkage(s), between mitochondria and microtubules possibly exists in these cells and is independent of the intermediate filaments.