Are Dihydroxyphenylalanine Decarboxylase and 5‐Hydroxytryptophan Decarboxylase Individual Enzymes?
- 8 December 1960
- journal article
- Published by Wiley in Acta Physiologica Scandinavica
- Vol. 49 (4) , 364-369
- https://doi.org/10.1111/j.1748-1716.1960.tb01958.x
Abstract
Using an enzyme preparation from rabbit kidney cortex, the amino acids 3,4‐dihydroxyphenylalanine and 5‐hydroxytryptophan were found to inhibit each other's enzymatic decarboxylation competitively. Competitive inhibitions of these reactions by m‐tyrosine, o‐tyrosine, and caffeic acid were also observed. After purification of an enzyme preparation on a column of DEAE cellulose a peak appeared which contained most of the dihydroxyphenylalanine decarboxylase activity present in the extract. In this peak also nearly all the 5‐hydroxytryptophan decarboxylase activity was found. The ratio of the two activities was practically the same throughout the peak. The data were in agreement with the assumption that dihydroxyphenylalanine and 5‐hydroxytryptophan were decarboxylated by one enzyme.Keywords
This publication has 12 references indexed in Scilit:
- On the distribution in brain of monoamines and of enzymes responsible for their formationCellular and Molecular Life Sciences, 1959
- Studies on 5-hydroxytryptophan decarboxylase. I. In vitro inhibition and substrate interactionArchives of Biochemistry and Biophysics, 1959
- Über die Reinigung und Spezifität der DOPA-DecarboxylaseHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1959
- CHROMATOGRAPHY OF SERUM PROTEINS IN NORMAL AND PATHOLOGIC SERA: THE DISTRIBUTION OF PROTEIN-BOUND CARBOHYDRATE AND CHOLESTEROL, SIDEROPHILIN, THYROXIN-BINDING PROTEIN, B12-BINDING PROTEIN, ALKALINE AND ACID PHOSPHATASES, RADIOIODINATED ALBUMIN AND MYELOMA PROTEINSJournal of Clinical Investigation, 1958
- Hemmung der Serotoninbildung durch ?-Methyl-DopaNaunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie, 1958
- 5-HYDROXYTRYPTOPHAN DECARBOXYLASE: PREPARATION AND PROPERTIESJournal of Biological Chemistry, 1954
- 5-HYDROXYTRYPTOPHAN DECARBOXYLASE: A NEW ROUTE OF METABOLISM OF TRYPTOPHANJournal of the American Chemical Society, 1953
- PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENTJournal of Biological Chemistry, 1951
- Fermentativer Abbau von l-Dioxyphenylalanin (Dopa) durch NiereNaunyn-Schmiedebergs Archiv für experimentelle Pathologie und Pharmakologie, 1938
- The Determination of Enzyme Dissociation ConstantsJournal of the American Chemical Society, 1934