Ribosomal RNA genes and the B chromosome of Brachycome dichromosomatica
- 1 May 1995
- journal article
- Published by Springer Nature in Heredity
- Vol. 74 (5) , 556-561
- https://doi.org/10.1038/hdy.1995.77
Abstract
Fluorescence in situ hybridization (FISH) with biotinylated rDNA revealed the presence of an rRNA gene cluster on both the A and B chromosomes of Brachycome dichromosomatica, an Australian native ephemeral plant of the arid regions of south-eastern Australia. This species contains only two pairs of A chromosomes and up to three B chromosomes. The regular attachment of the B chromosome to a nucleolus suggests that these ribosomal RNA genes are transcribed. Southern hybridization of DNA from 0B and +B plants digested with a variety of restriction enzymes indicates that the rRNA genes on the A and B chromosomes are the same in sequence and methylation status.Keywords
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