Kinetics of x-ray and heat inactivation of phi-x174 RF-DNA.
- 1 August 1965
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 54 (2) , 551-558
- https://doi.org/10.1073/pnas.54.2.551
Abstract
RF-DNA of X174 is inactivated by X-rays and heat with a killing efficiency of about 0.25, suggesting that the genome is made up of critical and non-critical targets. It appears that one lesion alone in the critical target kills the viral activity, while surviving RF bearing several lesions only in the noncritlcal target can still restitute a normal replica of the viral genome. Restitution mechanisms are discussed.Keywords
This publication has 12 references indexed in Scilit:
- Mechanisms of Inactivation of Single-Stranded Virus Nucleic Acids by HeatNature, 1964
- The effect of X-irradiation on the priming ability of DNA in the RNA polymerase systemBiochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects, 1964
- The u.v.-resistance of double-stranded φX174 DNAJournal of Molecular Biology, 1964
- Electron Microscopy of the Replicative Form of the DNA of the Bacteriophage ϕX174Science, 1963
- THE CYCLIC HELIX AND CYCLIC COIL FORMS OF POLYOMA VIRAL DNAProceedings of the National Academy of Sciences, 1963
- Radiosensitive Molecular Weight of Single-Stranded Virus Nucleic AcidsNature, 1963
- The process of infection with bacteriophage φX174: I. Evidence for a “Replicative form”Journal of Molecular Biology, 1962
- SELECTIVE HEAT INACTIVATION OF PNEUMOCOCCAL TRANSFORMING DEOXYRIBONUCLEATEProceedings of the National Academy of Sciences, 1961
- MECHANISMS OF INACTIVATION OF DEOXYRIBONUCLEIC ACIDS BY HEATProceedings of the National Academy of Sciences, 1961
- Fragmentary transfer of P32-labeled parental DNA to progeny phageVirology, 1961