Flash reactivation of Tris-inactivated chloroplasts
- 1 August 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant and Cell Physiology
- Vol. 19 (5) , 895-900
- https://doi.org/10.1093/oxfordjournals.pcp.a075665
Abstract
The light conditions required for reactivation of the oxygen-evolving system in Tris-treated chloroplasts were studied by means of repetitive flashes. Inactive Tristreated chloroplasts were washed with reduced 2,6-dichlorophenolindophenol, suspended in a reactivation medium containing Mn2++ and Ca2++ ions, then illuminated with flashes. Flashes at dark intervals of 2 sec were most effective for reactivation, while those at shorter or longer intervals were less effective. It was deduced that more than two sequential light reactions with dark reactions in between were involved in the reactivation.Keywords
This publication has 2 references indexed in Scilit:
- Light-dependent development of thermoluminescence, delayed emission and fluorescence variation in dark-grown spruce leavesBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1976
- Photoactivation of Oxygen‐evolving System in Dark‐grown Spruce SeedlingsPhysiologia Plantarum, 1976