Iodination of Escherichia coli with chloramine T: selective labeling of the outer membrane lipoprotein
- 1 May 1977
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 130 (2) , 775-780
- https://doi.org/10.1128/jb.130.2.775-780.1977
Abstract
Iodination of E. coli cells with chloramine T preferentially labels the free and murein-bound forms of the outer membrane lipoprotein. Iodination for 15 s at 15.degree. C labels the 2 forms of the lipoprotein almost exclusively, but iodination for 60 s at 25.degree. C also labels the other major outer membrane proteins. Chloramine T iodination is a rapid, simple technique for [radio-]labeling the outer membrane lipoprotein.This publication has 25 references indexed in Scilit:
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