Inhibition of Renal Prostaglandin Synthesis and Metabolism by Indomethacin in Rats

Abstract
The effect of indomethacin and other NSAID on renal prostaglandin synthesis and metabolism was studied in nondiuretic rats prepared for acute experimentation. Thirty minutes after the administration of a 2 mg/kg iv dose of indomethacin, the concentration of prostaglandin in renal venous blood as determined by bioassay was reduced 69%. In addition, conversion of radiolabeled arachidonic acid to prostaglandin E2 in vitro by the renal papillae of indomethacin pretreated rats was inhibited 97%.