The Contribution of the α and β Chains to the Kinetics of Oxygen Binding to and Dissociation from Hemoglobin
- 1 January 1973
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 70 (1) , 1-4
- https://doi.org/10.1073/pnas.70.1.1
Abstract
A new type of experiment in which hemoglobin is exposed briefly to oxygen has shown that the half-time of dissociation of oxygen from some partly oxygenated intermediates is about 1 msec at 20 degrees and 10 msec at 2 degrees . The rapid dissociation occurs selectively from one type of chain, provisionally identified as the beta-chain. Chains that show the rapid rate of dissociation of oxygen also bind rapidly. It follows that the kinetic equivalent of the Adair equation and the Monod-Wyman-Changeux model are quite unsuited to represent the kinetics of the oxygen-hemoglobin reaction. The reaction of oxygen with hemoglobin closely resembles that of the alkyl isocyanides and differs radically from that of carbon monoxide.Keywords
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