G1-PHASE ARREST OF CULTURED HUMAN-LEUKEMIC T-CELLS INDUCED BY DEOXYADENOSINE
- 1 January 1981
- journal article
- research article
- Vol. 41 (12) , 5141-5150
Abstract
Cultured human T-cell leukemic lymphocytes have enhanced sensitivity to growth inhibition by deoxyadenosine. Flow cytometry was used to investigate the mechanism of deoxyadenosine toxicity in cultured T-leukemic cells. Comparative studies on deoxyadenosine-resistant Epstein-Barr virus-transformed B-lymphocyte cell lines were also performed. After exposure of T-cells to low concentrations of deoxyadenosine (3 .mu.M), in the presence of an adenosine deaminase inhibitor (erythro-9-[3-(2-hydroxynonyl)]adenosine), accumulation of cells with a G1 DNA content was demonstrated. B-cell lines showed a similar degree of growth inhibition after exposure to 200-400 .mu.M deoxyadenosine but were blocked in S phase. The T-cell G1 block was associated with a rise in the deoxyadenosine triphosphate pool, and both these phenomena were prevented by the addition of deoxycytidine. The biochemical mechanism of this G1 block induced by deoxyadenosine in T-cells is not understood.This publication has 14 references indexed in Scilit:
- REMISSION INDUCTION WITH ADENOSINE-DEAMINASE INHIBITOR 2'-DEOXYCOFORMYCIN IN THY-LYMPHOBLASTIC LEUKÆMIAThe Lancet, 1980
- SENSITIVITY OF LEUKEMIC HUMAN NULL LYMPHOCYTES TO DEOXYNUCLEOSIDES1980
- Biochemical basis for differential deoxyadenosine toxicity to T and B lymphoblasts: role for 5'-nucleotidase.Proceedings of the National Academy of Sciences, 1979
- Biochemical basis for the enhanced toxicity of deoxyribonucleosides toward malignant human T cell lines.Proceedings of the National Academy of Sciences, 1979
- Characterization of a mutant mouse lymphoma cell with deficient transport of purine and pyrimidine nucleosides.Journal of Biological Chemistry, 1979
- Apparent suicide inactivation of human lymphoblast S-adenosylhomocysteine hydrolase by 2'-deoxyadenosine and adenine arabinoside. A basis for direct toxic effects of analogs of adenosine.Journal of Biological Chemistry, 1979
- S -Adenosylhomocysteine Hydrolase Is an Adenosine-Binding Protein: a Target for Adenosine ToxicityScience, 1978
- Deoxyadenosine metabolism and cytotoxicity in cultured mouse T lymphoma cells: a model for immunodeficiency diseaseCell, 1978
- Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system.Journal of Histochemistry & Cytochemistry, 1977
- Deoxycytidine kinase from calf thymus. Substrate and inhibitor specificity.Journal of Biological Chemistry, 1976