Influence of Serum, Estrogen, and Gonadotropins Upon Growth and Progesterone Secretion by Cultures of Granulosa Cells from Small Porcine Follicles
- 1 January 1976
- journal article
- research article
- Published by Taylor & Francis in Endocrine Research Communications
- Vol. 3 (5) , 319-333
- https://doi.org/10.1080/07435807609052936
Abstract
Granulosa cells from small (< 2mm) antral porcine follicles were grown in culture to study the effects of various hormones on growth, morphology and progesterone secretion. Culture medium 199D + 4% serum was found to be most suitable since it maintained a fairly constant cell population. Estradiol (lug/ml) and human FSH stimulated cell growth. LH and FSH stimulated progesterone secretion and induced morphological changes associated with luteinization. Estradiol (0.1ng/ml) inhibited progesterone secretion by granulosa cells.Keywords
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