Preferential usage of JH2 in D-J joinings with DQ52 in murine lymphocytes

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Abstract
Southern blot analysis of genomic DNA of normal mouse thymocytes with a JH4 probe, a probe for JH4 of Igh genes, consistently showed a second band in addition to a germiine band. The same band was also observed in analysis with a 5' DQ probe, a 5'-flanking sequence of DQ52, thus suggesting the use of DQ52. By analyzing EcoRI- and Pvull-digested genomic DNA, the above-observed band was demonstrated to be the result of DQ52-JH2 joining. This was further confirmed utilizing a T cell leukemia line with known DQ52-JH joinings. We then quantitatively estimated the frequencies of JH segment usages in joining with DQ52, through plaque hybridization assays. Three hundred and fifty JH4-positive clones were obtained from 7 × 105 plaques by plaque hybridization. Forty-eight randomly selected non-germline clones were purified and the use of JH segments was determined through Southern blot analysis. Nine clones used JH2, JH1 29 used JH2, 9 used JH3, and 1 used JH4, thus indicating an apparent preferential usage of JH2 segment. Southern blot analysis of genomic DNA of progenitor B cells in culture showed a dominant band of DQ52-JH2 joining 1 week after initiation of culture. This may indicate that the dominant DQ52-JH2 joining in thymocytes is representative of early D- J joinings in progenitor B cells.

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