Studies on the Normal Serum Panagglutinin Active Against Trypsinated Human Erythrocytes

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Abstract
20 subjects selected at random all contained panagglutinin in their serum active against their own trypsinated erythrocytes. The agglutination was reversed upon incubation and addition of NaCN. They regained their agglutinability in fresh serum and after washing the cells with saline. The cyanide reversal was shown to be accompanied by elution of the agglutinin. Heated serum and urine had no agglutinating activity but caused reversal of washed agglutinated cells with normal activity. Cells incubated with urine or with inactivated serum did not agglutinate on adding fresh serum but agglutinability was restored by washing. The factors involved in the agglutinin and reversion seem to compete for the red cell surface.