EINE EINFACHE SPEZIFISCHE ROUTINEMETHODE ZUR FLUOROMETRISCHEN BESTIMMUNG VON UNKONJUGIERTEN 11-HYDROXYCORTICOSTEROIDEN IN KÖRPERFLÜSSIGKEITEN

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Abstract
A highly improved specificity is obtained by the introduction of a simple correction in the fluorometric determination of unconjugated 11-hydroxy-corticosteroids (11-OHCS) in body fluids. The correction is based on the different development of fluorescence with time of specific and nonspecific fluorogens in alcoholic sulphuric acid. The specificity of the routine method is examined by comparison with a paperchromatographic technique. Due to our findings the corrected fluorogens of this simple routine method consist nearly of cortisol and corticosterone only. The additional reliability criteria (accuracy, precision and sensitivity) and the practicability of the method are described.