D1′ centers are less efficient than normal photosystem II centers
- 21 August 2001
- journal article
- Published by Wiley in FEBS Letters
- Vol. 505 (1) , 113-117
- https://doi.org/10.1016/s0014-5793(01)02794-6
Abstract
One prominent difference between the photosystem II (PSII) reaction center protein D1′ in Synechocystis 6803 and normal D1 is the replacement of Phe-186 in D1 with leucine in D1′. Mutants of Synechocystis 6803 producing only D1′, or containing engineered D1 proteins with Phe-186 substitutions, were analyzed by 77 K fluorescence emission spectra, chlorophyll a fluorescence induction yield and decay kinetics, and flash-induced oxygen evolution. Compared to D1-containing PSII centers, D1′ centers exhibited a 50% reduction in variable chlorophyll a fluorescence yield, while the flash-induced O2 evolution pattern was unaffected. In the F186 mutants, both the P680+/QA− recombination and O2 oscillation pattern were noticeably perturbedKeywords
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