Separation of Isomeric Insect Pheromonal Compounds Using Reversed-Phase HPLC with AgNO3 in the Mobile Phase

1 January 1981

journal article
research article
Published by Oxford University Press (OUP) in Journal of Chromatographic Science

Vol. 19 (1) , 13-17
https://doi.org/10.1093/chromsci/19.1.13

Abstract

Using reversed-phase HPLC and a polar mobile phase containing silver nitrate, rapid separations of geometrical isomers of mono-, di-, and polyunsaturated open chain olefins were achieved. These olefins represented various chain lengths of acetates, alcohols, aldehydes, and hydrocarbons. Separations of adjacent positional isomers of dodecenyl acetate were also attempted; the degree of success was dependent on double bond distance from the acetate group. Advantages of the mobile Ag⁺/reversed-phase technique over commonly used methods for geometrical isomer separation are discussed.

Keywords

REVERSED PHASE
OLEFINS
NITRATE
POLAR MOBILE
PHASE HPLC
GEOMETRICAL ISOMERS
PHASE CONTAINING
POLYUNSATURATED

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