Simplified method for conjugating macrocyclic bifunctional chelating agents to antibodies via 2-iminothiolane
- 1 May 1990
- journal article
- research article
- Published by American Chemical Society (ACS) in Bioconjugate Chemistry
- Vol. 1 (3) , 222-226
- https://doi.org/10.1021/bc00003a007
Abstract
A one-step method for conjugating macrocyclic chelators to antibodies using the protein modification reagent 2-iminothiolane controls aggregation, maintains immunoreactivity, and produces consistent chelate/antibody ratios. Conjugation conditions have been investigated with the macrocyclic chelates 6-[p-(bromoacetamido)benzyl]-1,4,8,11-tetraazacyclotetradecane-N,N',N '',N'''-tetraacetic acid and 2-[p-(bromoacetamido)benzyl]-1,4,7,10-tetraazacyclododecane-N,N',N '',N'''-tetraacetic acid, with three different monoclonal antibodies. The bifunctional chelating agents are prepared by bromoacetylation of their amine precursors using a two-phase H(2)O/CHCl(3) system, which improves product purity.This publication has 8 references indexed in Scilit:
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