Gemeinsame Präparation von drei Dehydrogenasen und zwei Kinasen in einem Arbeitsgang aus Schweineherz / The Preparation of Three Dehydrogenases and Two Kinases from Pig Heart by a Single Procedure
Open Access
- 1 April 1977
- journal article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 32 (3-4) , 210-218
- https://doi.org/10.1515/znc-1977-3-410
Abstract
A single procedure for the preparation of lactate dehydrogenase (EC 1.1.1.27), the mitochondrial and cytoplasmic forms of malate dehydrogenase (EC 1.1.1.37), adenylate kinase (EC 2.7.4.3) and pyruvate kinase (EC 2.7.1.40) from pig heart is described. The five enzymes are obtained in preparative amounts in homogenous form with specific activities equal to or higher than those pre viously reported. Some molecular properties of pig heart pyruvate kinase are determined.Keywords
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