Virologic Use of Monkey Kidney Cells Preserved by Freezing.

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Abstract
Cells of freshly trypsinized monkey kidney and of primary cultures of monkey kdney were preserved by slow freezing in 5% glycerol medium and storage at -75[degree]C. Cultures of thawed cells yielded monolayers that could be directly utilized for virologic work. In these, the cytologic response of plaque morphology due to polio-viruses, ECHO, Coxsackie, measles, herpes simplex, influenza, CA, HA-1, JH, 2060, and adenovirus, as well as assays by tube titration or plaque counts, were equivalent to those produced in unfrozen cells. Moreover, isolations of ECHO 9, Coxsackie A-9, B-5, and polioviruses, from stools or cerebrospinal fluids were made''with equal facility in in either type of culture.