A Rapid and Simple ABO Genotype Screening Method Using a Novel B/O2 versus A/O2 Discriminating Nucleotide Substitution at the ABO Locus
- 1 October 1995
- journal article
- research article
- Published by Wiley in Vox Sanguinis
- Vol. 69 (3) , 242-247
- https://doi.org/10.1111/j.1423-0410.1995.tb02602.x
Abstract
An ABO genotype screening method discriminating the common alleles A1, A2, B, O1 and O2 at the ABO locus was made possible by the discovery of a novel nucleotide substitution (G1096A) present only in B and O2 alleles. A rapid and reliable single‐tube approach using multiplex PCR with four primers amplifying exons 6 and 7 of the ABO genes followed by simultaneous addition of two restriction enzymes was developed and validated in a population of 150 Swedish blood donors. This technique is the most cost‐efficient and informative ABO genotyping method reported to date.Keywords
This publication has 0 references indexed in Scilit: