The Establishment of Three Cell Lines from the Tick Rhipicephalus Appendiculatus (Agari: Ixodidae) and their Infection with Some Arboviruses
- 10 January 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Medical Entomology
- Vol. 11 (6) , 698-706
- https://doi.org/10.1093/jmedent/11.6.698
Abstract
The establishment of 3 cell lines from developing adult tissues of Rhipicephalus appendiculatus is described. The cell lines are currently (March 1974) at the 54th, 41st and 34th subculture level and are grown in Leibovitz L-15 medium supplemented with 10% tryptose phosphate broth and 10% fetal calf serum. Cells from one of the lines were successfully infected with West Nile, Louping ill, Langat and Quaranfil viruses.Keywords
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