Correlation between the 32-kDa sigma factor levels and in vitro expression of Escherichia coli heat shock genes.
- 1 December 1987
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 84 (23) , 8365-8369
- https://doi.org/10.1073/pnas.84.23.8365
Abstract
S-30 extracts from Escherichia coli cells were used to express heat shock (HS) and non-HS genes in vitro in a DNA-directed protein synthesis system. The S-30 extracts prepared from cells that have been shifted to 45.degree.C express HS genes in vitro .apprxeq. 8 times better than extracts from cells at 33.degree.C. In contrast, the expression of non-HS genes in extracts from heat-induced cells is only 40% of that seen in extracts from cells at 33.degree.C. These results correlate well with the levels of HS .sigma. factor and normal .sigma. factor bound to RNA polymerase. Thus, there was an 8-fold increase in the HS .sigma. factor and a 60% decrease in the normal .sigma. factor associated with RNA polymerase at the higher temperature. Part of the increase in the level of the HS .sigma. factor could be accounted for by a 3-fold increase in the level of HS .sigma. factor mRNA during heat induction.This publication has 25 references indexed in Scilit:
- The heat shock response of E. coli is regulated by changes in the concentration of σ32Nature, 1987
- Induction of the heat shock response of E. coli through stabilization of sigma 32 by the phage lambda cIII protein.Genes & Development, 1987
- Transcription from a heat-inducible promoter causes heat shock regulation of the sigma subunit of E. coli RNA polymeraseCell, 1984
- Nucleotide sequence of the heat shock regulatory gene of E. coli suggests its protein product may be a transcription factorCell, 1984
- Effects of the mutant sigma allele rpoD800 on the synthesis of specific macromolecular components of the Escherichia coli K12 cellJournal of Molecular Biology, 1984
- Control of phage λ development by stability and synthesis of cll protein: Role of the viral clll and host hflA, himA and himD genesCell, 1982
- Positive regulatory gene for temperature-controlled proteins in Escherichia coliBiochemical and Biophysical Research Communications, 1981
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Procedure for the rapid, large-scale purification of Escherichia coli DNA-dependent RNA polymerase involving polymin P precipitation and DNA-cellulose chromatographyBiochemistry, 1975
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970