CHROMOSOME MARKER EVIDENCE FOR THE BIPOTENTIALITY OF BFU-E

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 56  (2) , 318-322
Abstract
When mouse bone marrow cells are seeded in agar cultures containing erythropoietin or pokeweed mitogen-stimulated spleen cell conditioned medium plus erythropoietin, megakaryocytes are found mixed with erythroid cells in approximately 40% of the erythropoietic bursts that develop in the cultures. Chromosome spreads of C-metaphases in these megaerythro bursts were prepared and stained in situ with a modification of the C-banding technique. In cultures seeded with mixtures of male and female cells, metaphases from individual megakaryoctes were of the same sex as diploid C-metaphases of erythroid cells in the same megaerythro burst. In the mouse, a bipotential progenitor cell exists that has the capacity to give rise to cells of both the megakaryocytic and the erythrocytic lines of differentiation.