ELECTROPHORETIC ANALYSIS OF COLORED PRODUCT PRODUCED BY SCHIFF-FORMALDEHYDE REACTION

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Abstract
The colored mixture of Schiff reagent and formaldehyde was analysed by paper electrophoresis (Medium; Pyridine acetic acid buffer, pH 3.4, current; 5.7-6.4 ma/8 cm, voltage; 500 v/27 cm. Duration; 25 minutes). The electrophoresis revealed the existence of as many as 10 components, at a maximum, in the mixture. The test for partition of these components between i-amylol and water showed that among them only 6 components were the genuine reaction products. It was shown also that only 3 among these products were resistant to reduction by SO2, and responsible for the reaction color in routine procedures. The number and the relative amounts of these components changed according to the added amounts of formaldehyde as well as the time of reaction. All these facts indicate that the mechanism of Schiff -formaldehyde reaction is much more complicated than that formerly assumed by Wieland and Scheuing (Ber. chem. Ges., 1921, 54, 2527).

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