Capillary electrophoresis of isoquinoline alkaloids from Chelidonium Majus L.

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Abstract
The determination of six different isoquinoline alkaloids from Chelidonium majus (leaves and roots) using capillary electrophoresis is described. Analyses were performed on fused silica capillaries with 66 mM phosphate buffer at pH 2.4 containing 40% methanol. The analytes were monitored by on‐line ultraviolet (UV) detection to identify sanguinarine, chelerythrine, berberine and chelidonine. Both coptisine and DL‐stylopine were isolated by semi‐preparative high performance liquid chromatography (HPLC), and their structure assessed by UV and mass spectrometry. Capillary electrophoresis is confirmed as a useful complementary technique to HPLC.