Establishment of a human pancreatic cancer cell line and detection of pancreatic cancer associated antigen.

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Abstract
A human pancreatic cancer cell line (PK-1) was established from liver metastasis of pancreatic cancer. Primary pancreatic cancer cells grew as islands surrounded by fibroblastic cells. However, these fibroblastic cells were gradually omitted by the polygonal shaped cancer cells. This cell line contained neither zymogen granules nor trypsin indicating that this pancreatic cancer originated from pancreatic duct cells. Modal chromosome numbers of this cell line were 42 and 72 and the doubling time was 48 h. This cell line was transplantable in athymic nude mice to form progressive tumors which had histology similar to that of the original cancer (papillotubular adenocarcinoma). CEA [carcinoembryonic antigen] was detected on the surface and in the cytoplasm of this cell line by indirect immunofluorescence while ferritin and .alpha.-fetoprotein were not found. Rabbit antiserum against this pancretic cancer cell line detected pancreatic cancer associated antigen besides CEA in the culture supernatant. This antiserum reacted with sera from patients with pancreatic cancer to form a distinct precipitin line in agrose gel which fused with the precipitin line formed between the culture supernatant of this cell line and the antiserum.

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