MOLECULAR COMPARATIVE STUDIES AMONG BLASTOCYSTIS ISOLATES OBTAINED FROM HUMANS AND ANIMALS
- 1 June 2003
- journal article
- research article
- Published by American Society of Parasitologists in Journal of Parasitology
- Vol. 89 (3) , 585-594
- https://doi.org/10.1645/0022-3395(2003)089[0585:mcsabi]2.0.co;2
Abstract
This study compared specific polymerase chain reaction (PCR) primers and phylogenetic tree analysis of restriction fragment length polymorphism using the small subunit ribosomal RNA gene in various Blastocystis populations. A phylogenetic tree was constructed using 12 restriction enzymes and a sample pool of 22 isolates, including 2 reference strains and Proteromonas lacertae as an outgroup. The analysis showed that the 22 isolates could be separated into 7 clusters. Four of the 7 clusters were mixed groups that comprised isolates from both humans and nonhuman hosts. The other 3 clusters contained isolates from humans or nonhuman hosts only. The phylogenetic analysis also showed that B. hominis isolates from geographical separated areas did not necessarily cluster in the genetically different groups. The results of genetic homology and phylogenetic tree analysis among Blastocystis isolates from humans and animals indicated that all isolates from animals appear to be B. hominis. Polymerase chain reaction amplifications using previously described and newly defined specific primers mirrored the clusters obtained by the phylogenetic tree analysis. Our results show that primer PCR can be used as a powerful tool for the typing of Blastocystis populations.Keywords
This publication has 21 references indexed in Scilit:
- Use of the elongation factor-1α gene in a polymerase chain reaction-based restriction-fragment-length polymorphism analysis of genetic heterogeneity among Blastocystis speciesMolecular and Biochemical Parasitology, 2001
- Restriction-fragment-length polymorphism analysis of small-subunit rRNA genes of Blastocystis hominis isolates from geographically diverse human hostsZeitschrift Fur Parasitenkunde-Parasitology Research, 2000
- Structural basis of karyotype heterogeneity in Giardia lambliaMolecular and Biochemical Parasitology, 1998
- Karyotypic diversity among Blastocystis hominis isolatesInternational Journal for Parasitology, 1997
- Five subgroups of Blastocystis hominis isolates from symptomatic and asymptomatic patients revealed by restriction site analysis of PCR‐amplified 16S‐like rDNATropical Medicine & International Health, 1997
- Riboprinting: A Tool for the Study of Genetic Diversity in Microorganisms1The Journal of Eukaryotic Microbiology, 1997
- Extensive genetic diversity in Blastocystis hominisMolecular and Biochemical Parasitology, 1997
- Description of a Blastocystis species from Rattus norvegicusZeitschrift Fur Parasitenkunde-Parasitology Research, 1997
- Human parasite finds taxonomic homeNature, 1996
- Axenic culture of reptilian Blastocystis isolates in monophasic medium and speciation by karyotypic typingZeitschrift Fur Parasitenkunde-Parasitology Research, 1996