Multiplex genotyping of PCR products with MassTag-labeled primers.
Open Access
- 1 September 1997
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 25 (18) , 3749-3750
- https://doi.org/10.1093/nar/25.18.3749
Abstract
A simple mass spectrometric based assay, the PinPoint assay, has previously been described for typing single nucleotide polymorphisms. The identity of a polymorphism is determined by mass differences of single base extended genotyping primers as determined by MALDI-TOF mass spectrometry. A simple method for multiplexing the assay is described, employing multiple primers with 5'oligo(dT) sequences (MassTags) which serve to mass discriminate the peaks of multiple extended and non-extended primers. The assay is extremely rapid and requires no labeling reagents.Keywords
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