(+)‐Tubocurarine is a potent inhibitor of cation channels in the vacuolar membrane of Chenopodium rubrum L
- 17 December 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 277 (1-2) , 220-222
- https://doi.org/10.1016/0014-5793(90)80849-e
Abstract
The effect of the acetylcholine antagonist and channel blocker (+)-tubocurarine on the calcium-dependent slow vacuolar (SV) cation channels in the tonoplast of suspension-cultured cells of Chenopodium rubrum L. was examined using the patch-clamp technique. In whole-vacuolar recordings the drug strongly suppressed the potassium conductance (EC50: 6 μM) and altered the kinetics of channel inactivation. In excised membrane patches (+)-tuboeurarine evokes channel-‘flickering’ without affecting the single-channel conductance (approx. 80 pS).Keywords
This publication has 10 references indexed in Scilit:
- Potassium ion channels in the plasmalemmaPhysiologia Plantarum, 1990
- Two Proton Pumps Operate in Parallel Across the Tonoplast of Vacuoles Isolated from Suspension Cells ofChenopodium rubrumL.*Botanica Acta, 1989
- The Physiology of ION Channels and Electrogenic Pumps in Higher PlantsAnnual Review of Plant Biology, 1989
- Characterization of an anion-permeable channel from sugar beet vacuoles: effect of inhibitorsThe EMBO Journal, 1988
- Cytoplasmic calcium regulates voltage-dependent ion channels in plant vacuolesNature, 1987
- Single calcium‐activated potassium channels recorded from cultured rat sympathetic neurones.The Journal of Physiology, 1987
- Tubocurarine suppresses slow calcium‐dependent after‐hyperpolarization in guinea‐pig inferior mesenteric ganglion cells.The Journal of Physiology, 1986
- ATP-dependent acidification and tonoplast hyperpolarization in isolated vacuoles from green suspension cells of Chenopodium rubrum LProceedings of the National Academy of Sciences, 1986
- Effects of apamin, quinine and neuromuscular blockers on calcium‐activated potassium channels in guinea‐pig hepatocytes.The Journal of Physiology, 1985
- Tight-Seal Whole-Cell RecordingPublished by Springer Nature ,1983