Identification and characterization of a melanin-concentrating hormone receptor
Open Access
- 19 June 2001
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 98 (13) , 7576-7581
- https://doi.org/10.1073/pnas.131200698
Abstract
Melanin-concentrating hormone (MCH), a neuropeptide expressed in central and peripheral nervous systems, plays an important role in the control of feeding behaviors and energy metabolism. An orphan G protein-coupled receptor (SLC-1/GPR24) has recently been identified as a receptor for MCH (MCHR1). We report here the identification and characterization of a G protein-coupled receptor as the MCH receptor subtype 2 (MCHR2). MCHR2 has higher protein sequence homology to MCHR1 than any other G protein-coupled receptor. The expression of MCHR2 has been detected in many regions of the brain. In contrast to MCHR1, which is intronless in the coding region and is located at the chromosomal locus 22q13.3, the MCHR2 gene has multiple exons and is mapped to locus 6q21. MCHR2 is specifically activated by nanomolar concentrations of MCH, binds to MCH with high affinity, and signals through Gq protein. This discovery is important for a full understanding of MCH biology and the development of potential therapeutics for diseases involving MCH, including obesity.Keywords
This publication has 47 references indexed in Scilit:
- Isolation and Identification of Melanin-Concentrating Hormone as the Endogenous Ligand of the SLC-1 ReceptorBiochemical and Biophysical Research Communications, 1999
- Melanin-Concentrating Hormone Binding Sites in Human SVK14 KeratinocytesBiochemical and Biophysical Research Communications, 1997
- Structural basis of receptor/G protein coupling selectivity studied with muscarinic receptors as model systemsLife Sciences, 1997
- Minireview: Differential Targeting and Retention of G Protein-Coupled Receptors in Polarized Epithelial CellsJournal of Receptors and Signal Transduction, 1997
- Characterization of a human gene related to genes encoding somatostatin receptorsFEBS Letters, 1996
- Melanin‐concentrating hormone binding to mouse melanoma cells in vitroFEBS Letters, 1995
- Synthesis and lodination of human (phenylalanine13, tyrosine19) melanin‐concentrating hormone for radioreceptor assayJournal of Peptide Science, 1995
- Hidden Markov Models of the G-Protein-Coupled Receptor FamilyJournal of Computational Biology, 1994
- Alpha-MSH and MCH are functional antagonists in a CNS auditory gating paradigmPeptides, 1993
- Immunosuppressive activity in the series of cycloamanide peptides from mushroomsPeptides, 1993