TRANSFERRIN BINDING AND IRON UPTAKE BY MOUSE LYMPH-NODE CELLS DURING TRANSFORMATION IN RESPONSE TO CONCANAVALIN-A
- 1 January 1981
- journal article
- research article
- Vol. 43 (2) , 393-398
Abstract
Mouse lymph node cells cultured with concanavalin A (Con A) in serum-free medium containing 59Fe-transferrin [TF] took up 59Fe more rapidly than cells cultured without Con A. Uptake of Fe commended rapidly and preceded the DNA synthesis onset in stimulated cells. Total uptake of TF during culture was much < that of Fe, indicating that cells could remove Fe from TF. The released TF appeared to be undegraded. Lymphoblasts bound 6 times more TF per cell than small lymphocytes. Lymphocytes also took up Fe from citrate and nitrilotriacetate complexes, and Fe so acquired was not readily removed by deferrioxamine, indicating that it was not bound extracellulary.This publication has 16 references indexed in Scilit:
- Growth of a human mammary tumour cell line in a serum-free mediumNature, 1979
- Transferrin receptors on human B and T lymphosblastoid cell linesBiochimica et Biophysica Acta (BBA) - General Subjects, 1979
- Iron uptake by Chang cells from transferrin, nitriloacetate and citrate complexesThe effects of iron-loading and chelation with desferrioxamineBiochimica et Biophysica Acta (BBA) - General Subjects, 1978
- Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes.The Journal of Experimental Medicine, 1978
- Uptake of transferrin-bound zinc by human lymphocytesCellular Immunology, 1978
- Iron salts and transferrin are specifically required for cell division of cultured 3T6 cellsBiochemical and Biophysical Research Communications, 1977
- The iron-binding function of transferrin in iron metabolism.1977
- Effect of Iron Deficiency and Desferrioxamine on DNA Synthesis in Human CellsBritish Journal of Haematology, 1976
- Effect of iron transferrin on nucleic acid synthesis in phytohemagglutinin-stimulated human lymphocytesCellular Immunology, 1975
- Transferrin and iron uptake by human cells in cultureExperimental Cell Research, 1974