Differentiation of Herpetomonas megaseliae: Effects of Hydroxyurea on Morphology and Growth

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Abstract
H. megaseliae grown in the presence of hydroxyurea showed a decline in population during the first 24 h, eventually increasing slightly over the 7 day incubation. Throughout the 7 day incubation period the population of the treated organisms never equaled that of the control organisms. The percentage of promastigotes as opposed to para- and opisthomastigotes in the treated cultures did not differ from that in the control cultures through the first 3 days of incubation. Thereafter, the percentage of promastigotes in the treated cultures dropped significantly below that of the controls. At the light microscope level, the treated organisms showed an increase in size with associated striations at the anterior end. A multiflagellate (nondividing) condition was also observed in the hydroxyurea-treated organisms. T[transmission]EM and S[scanning]EM studies showed pellicular convolutions which were interpreted as the striations observed under the light microscope. The differences in population number, kinetoplast number and position and pellicular morphology between control and experimental cultures suggests that enhanced differentiation and abortive cytokinesis are the net effects of hydroxyurea on H. megaseliae.

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