Molecular forms and activities of glycosidases in cultures of amniotic-fluid cells

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Abstract
A growing number of inborn errors of metabolism can be diagnosed antenatally by analysis of enzyme activities in amniotic-fluid cells. Ion-exchange chromatography or gel filtration demonstrated the presence of different molecular forms of 9 lysosomal enzymes in cultured [human] amniotic-fluid cells. The patterns of molecular forms were similar to those known in skin fibroblasts and liver tissue. During cultivation total enzyme activities fluctuated with the number of passages, without a consistent trend, and without correlation to the dominating cell type in the culture.