PURIFICATION OF A HUMAN PROSTATE SPECIFIC ANTIGEN1

Top Cited Papers
Publisher Website
Google Scholar
Abstract
Rabbit antiserum raised against the crude extract of normal human prostatic tissue contained antibodies to a prostatic tissue-specific antigen as shown by immunoprecipitation techniques. Using this antiserum a prostate antigen was detected in normal, benign hypertrophic and malignant prostatic tissues, but not in other human tissues. The prostate antigen was purified to homogeneity from prostatic tissues and showed a single protein band on analytical polyacrylamide gel electrophoresis and isoelectric focusing. This report thus presents the 1st demonstration of the purification of a prostate-specific antigen that does not represent prostatic acid phosphatase. [The role of prostate antigen is prostatic cancer and its potential use for prostatic cancer diagnosis were discussed].