New Column Packing Materials for Separation of Water-Soluble Vitamins by High-Performance Liquid Chromatography

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Abstract
Three types of high-performance liquid chromatographic column packing materials ((3-morpholinopropyl)-, [3-(1-piperazinyl)propyl]-, and (3-piperidinopropyl)silyl silica gels) were newly prepared and examined for their ability to separate water-soluble vitamins. Six vitamins (ascorbic acid, nicotinic acid, thiamine hydrochloride, pyridoxine hydrochloride, hydroxocobalamin acetate, and flavin adenine dinucleotide) in sample solutions could be completely separated within less than 12 min by UV detection on a column packed with morpholinopropylsilyl silica gel by using a phosphate buffer [0.1 M (1 M=1 moldm−3) H3PO4−0.1 M Na2HPO4 (pH 6.0) containing 10 mM tetrabutylammonium bromide] as an eluent. This efficient gel was successfully applied to vitamin analyses of commercially available healthful beverages. The other column packing materials also showed characteristic for specific water-soluble vitamin analysis. Typical separations are presented.