Plasmid mapping computer program

11 January 1984

journal article
research article
Published by Oxford University Press (OUP) in Nucleic Acids Research

Vol. 12 (1Part2) , 717-729
https://doi.org/10.1093/nar/12.1part2.717

Abstract

Three new computer algorithms are described which rapidly order the restriction fragments of a plasmid DNA which has been cleaved with two restriction endonucleases in single and double digestions. Two of the algorithms are contained within a single computer program (called MPCIRC). The Rule-Oriented algorithm, constructs all logical circular map solutions within sixty seconds (14 double-digestion fragments) when used in conjunction with the Permutation method. The program is written in Apple Pascal and runs on an Apple II Plus Microcomputer with 64K of memory. A third algorithm is described which rapidly maps double digests and uses the above two algorithms as adducts. Modifications of the algorithms for linear mapping are also presented.

Keywords

This publication has 2 references indexed in Scilit:

Least-squares method for restriction mapping
Gene, 1978
Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis.
Proceedings of the National Academy of Sciences, 1977