Abstract
Special problems are inherent in the unambiguous location of sulphate esters in polysaccharides which contain a high proportion of 3,6-anhydrogalactose, because of the lability of this sugar residue and its glycosidic linkage. We have developed an oxidative hydrolysis of such polysaccharides to give Oligosaccharide sulphates in high yield and thus, by separation and characterisation, to establish the sulphate positions. In this way, the presence is confirmed in κ-carrageenan of 3-linked galactose 4-sulphate residues as the major sulphate ester, with smaller amounts of 4-linked 3,6-anhydrogalactose 2-sulphate and 4-linked galactose 6-sulphate.

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