A STUDY OF THE FRACTIONATION OF NEUTRAL URINARY STEROIDS BY ADSORPTION ON MAGNESIUMOXIDE

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Abstract
Purification of total neutral fractions of urinary steroids is necessary to overcome atypical colors caused by urinary pigments and chromogens in the Zimmerman and Pincus reactions. In the use of the Girard reagent a loss of 17-ketosteroids occurs, and ketonic fractions obtained are seldom free from pigment and nonketonic chromogens. A better method seems to be that of adsorption on MgO and elution with ether as described by Bauman and Metzger. Crude neutral urinary extracts from 23 healthy [male][male] were pooled and fractionated by this method. Fractions ob-tained, i.e., the petroleum ether insoluble residue, the frac-tion not adsorbed on magnesium oxide, the magnesium oxide eluate consisting principally of 17-ketosteroids, and the fraction not eluted with ether, were made up with 95% redistilled alc. to the original vol. of crude extract, and absorption curves were made of the colors produced by both Zimmerman and Pincus reagents. The color developed by the Zimmerman reagent with the MgO eluate could not be grossly distinguished from that of true 17-ketosteroids. Colors developed in the Pincus reaction were not visibly distinguishable from androsterone controls.

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