A Comparison of the Actions of Stimulatory Follicular Fluid and Gonadotropin-Releasing Hormone Analogs on Progesterone Secretion by Porcine Granulosa Cells1

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Abstract
Granulosa cells from small and medium porcine follicles (1-5 mm) were incubated with charcoal-treated follicular fluid from large (6-10 mm) follicles or porcine serum in the presence and absence of gonadotropin-releasing hormone (GnRH) analog and luteinizing hormone (LH) or follicle-stimulating hormone (FSH). A GnRH agonist inhibited follicular fluid''s enhancement of basal and LH-stimulated progesterone secretion but did not block follicular fluid''s enhancement of FSH-stimulated progesterone secretion. A GnRH antagonist mimicked follicular fluid''s enhancement of basal and LH-stimulated progesterone secretion but did not mimic follicular fluid''s action on FSH-stimulated progesterone secretion. When the GnRH antagonist and follicular fluid were added together, they acted synergistically in stimulating basal progesterone secretion, and were additive in enhancing LH-stimulated progesterone secretion. These observations suggest that separate follicular fluid molecules are responsible for its influence on LH and FSH actions on granulosa cells and that a GnRH-antagonist-like molecule could be responsible for some of follicular fluid''s "luteinization stimulatory" action. Alternatively, the stimulatory follicular fluid molecule may not resemble GnRH but may act via a mechanism that is opposed by GnRH.

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