Subcutaneous, Isogeneic Transplantation of Either Duct-Ligated Pancreas or Isolated Islets in Streptozotocin Diabetic Mice

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Abstract
Recovery from hyperglycemia was observed in streptozotocin diabetic mice that received s.c. isogeneic transplants of either isolated islets or duct-ligated pancreas. Transplants of isolated islets obtained from collagenase-digested adult pancreas provided recovery from hyperglycemia, but the incidence of recovery depended on the amount of islet tissue initially transplanted. Hyperplastic, insulin-rich islets obtained from the pancreas of obese hyperglycemic mice (ob/ob) allowed recovery between 3 and 6 wk, whereas an equivalent number of islets obtained from non-obese, normal donors gave only partial recovery after 8 wk. Implantation of pancreatic endocrine tissue obtained from adult donors whose pancreatic ducts were ligated several weeks earlier, led to consistent recovery within 8-10 wk. The content of immunoreactive insulin (IRI) extracted from transplants of mice recovering from hyperglycemia was 16-19% of that found in the normal mouse pancreas and was about 4 times greater than that remaining in the recipient''s own pancreas. Transplants removed from hosts that did not recover contained a relatively small amount of IRI indicating that these transplants contained insufficient insulin stores to allow recovery from hyperglycemia.

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