Serial Passage of Sheep Scrapie Inoculum in Suffolk Sheep
Open Access
- 1 January 2009
- journal article
- research article
- Published by SAGE Publications in Veterinary Pathology
- Vol. 46 (1) , 39-44
- https://doi.org/10.1354/vp.46-1-39
Abstract
Scrapie is a naturally occurring fatal neurodegenerative disease of sheep and goats. Susceptibility to the disease is partly dependent upon the genetic makeup of the host. In a recent study, it was shown that sheep intracerebrally inoculated with a US scrapie agent (No. 13–7) developed scrapie and survived for an average of 19 months post inoculation. In the present study, when this scrapie inoculum was further passaged for 3 successive generations, the survival time was reduced by approximately 8 months in scrapie-susceptible (QQ on prion protein gene [ PRNP] at codon 171) Suffolk sheep. It is concluded that inoculum No. 13–7 appears to have been stabilized in susceptible (171 QQ) Suffolk sheep and may be considered a specific isolate of sheep scrapie agent in the USA and therefore that it can be used to evaluate other isolates of sheep scrapie in this country.Keywords
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