Map-based cloning of chloronerva, a gene involved in iron uptake of higher plants encoding nicotianamine synthase
- 8 June 1999
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 96 (12) , 7098-7103
- https://doi.org/10.1073/pnas.96.12.7098
Abstract
The uptake of iron in plants is a highly regulated process that is induced on iron starvation. In tomato, the mutant chloronerva exhibits constitutive expression of iron uptake responses and intercostal chlorosis. Biochemically, chloronerva is an auxotroph for nicotianamine, a key polyamine in plant iron uptake metabolism. The chloronerva gene has been fine-mapped onto the long arm of chromosome 1 in a large segregating tomato population and yeast artificial chromosome clones encompassing the region were isolated by using flanking markers. A cosmid contig containing the chloronerva gene was established, and complementing cosmids were identified by transformation into the mutant. The chloronerva transcript was identified by cDNA isolation using the complementing cosmids. The gene encodes a unique protein of 35 kDa. The mutant harbors a single base change compared with the wild type. Based on enzyme activity and sequence similarity to the coding DNA sequence of the purified barley enzyme the chloronerva gene encodes the enzyme nicotianamine synthase.Keywords
This publication has 28 references indexed in Scilit:
- Cloning of Nicotianamine Synthase Genes, Novel Genes Involved in the Biosynthesis of PhytosiderophoresPlant Physiology, 1999
- Iron transport and storage in plantsTrends in Plant Science, 1997
- Absence of nicotianamine synthase activity in the tomato mutant ‘chloronerva’Journal of Plant Nutrition, 1996
- Nucleotide Sequence of a cDNA Encoding a Heat-Shock Protein (HSP70) from Barley (Hordeum vulgare L.)Plant Physiology, 1994
- Nicotianamine: mediator of transport of iron and heavy metals in the phloem?Physiologia Plantarum, 1993
- Nicotianamine ‐ a common constituent of strategies I and II of iron acquisition by plants: A reviewJournal of Plant Nutrition, 1992
- Biosynthesis of PhytosiderophoresPlant Physiology, 1990
- Different strategies for iron acquisition in higher plantsPhysiologia Plantarum, 1987
- Nicotianamine, a possible phytosiderophore of general occurrencePhytochemistry, 1980
- Versuche zur Normalisierung des Phänotyps der Mutantechloronerva vonLycopersicon esculentum MillGenetic Resources and Crop Evolution, 1960