Characterization of new enkephalin‐containing peptides in the adrenal medulla by immunoblotting
- 9 July 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 172 (2) , 303-308
- https://doi.org/10.1016/0014-5793(84)81146-1
Abstract
Immunoblotting combined with radioimmunoassays (RIAs) directed specifically towards certain sequences of the proenkephalin molecule has been used to characterize the enkephalin-containing peptides (ECPs) present in the bovine adrenal medulla. Immunoblotting allowed the simultaneous visualization of all ECPs present in a crude extract of this gland. Combining this technique with RIAs we have been able to characterize a new high molecular mass ECP, a 23.3-kDa protein which contains the amino-terminal part of proenkephalin and ends with the sequence of Leu-enkephalin at its carboxy-terminusKeywords
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